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Cryogrinder For Pharmaceuticals

Fat pad and nipple tissues were homogenized using a CryoGrinder OPS Diagnostics. Metabolites were extracted using cold acetonitrile-water 8020 vv. Five hundred microliter of extraction buffer was added to 20 mg of tissue, shaken vigorously for 2 min, and centrifuged 10,000 rpm 5 min . The supernatant was collected, and the.

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